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. 2018 Apr 26;42(1):299–308. doi: 10.3892/ijmm.2018.3645

Figure 2.

Figure 2

Mid-dose BSNXD administration significantly inhibited the OVX-induced increases of CD4+ T cells in the spleen and BM. Following 8 weeks of BSNXD administration, mice in the six experimental groups were sacrificed. The BM was then flushed out and single cells were isolated from the spleen by means of density (1,084±0,0010 g/ml) gradient centrifugation and then labelled with fluorescent antibodies to examine the percentage of CD4+ T cells using flow cytometry. (A) Images of the quantification of CD4+ T cells in the spleen in each group by flow cytometry. (B) Percentage of CD4+ T cells in the spleen or BM. Data are expressed as the mean ± standard error of the mean. **P<0.01 vs. OVX. BSNXD, Bu-Shen-Ning-Xin Decoction; OVX, ovariectomized; E2, estradiol; BM, bone marrow; CD, cluster of differentiation.