. 2018 Mar 26;8:5205. doi: 10.1038/s41598-018-23482-4

Table 1.

In vitro inhibitory potencies of synthesized benzylidenebarbituric acid derivatives.

Compound		IC₅₀ (nM)^a against mPGES-1		COX-1/2 Inhibition (%) at 100 µM^b
ID	X	Human mPGES-1	Mouse mPGES-1	COX-1/2 Inhibition (%) at 100 µM^b
4a	H	622 ± 121	7080 ± 627	0 ± 15
4b	Cl	33 ± 3	157 ± 31	4.3 ± 6.0
4c	Br	45 ± 8	917 ± 321	63 ± 0.8
4d	Me	82 ± 10	18900 ± 4080	9.1 ± 6.6
4e	OH	116 ± 17	2900 ± 293	68 ± 3.4
4f	OMe	121 ± 20	146 ± 209	28 ± 3.6
4g	OEt	186 ± 26	2410 ± 339	0 ± 15.1
4h	NO₂	67 ± 20	698 ± 97	43 ± 3.2
4i		87 ± 27	19100 ± 3490	56 ± 3.7
6		69 ± 16	292 ± 47	17 ± 4.6

^aData are expressed as the Mean ± SD of measurements in triplicate. ^bThe % inhibition of the compound at a concentration of 100 µM against the COX-1/2 (with equal amounts of COX-1 and COX-2 in terms of the enzyme activities).