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. 2018 Mar 26;8:5192. doi: 10.1038/s41598-018-23514-z

Figure 4.

Figure 4

Gene expression of a S. venezuelensis Cytoplasmic WAGO in each developmental stage. Median expression levels calculated from biological replicates (three replicates for PF and FLF; two replicates for all other life cycle stages) for a WAGO-like coding gene (GenBank ID: LM524968.1) upregulated in the parasitic adult female (PF) compared with free-living adult females (FLF) stage of S. venezuelensis (red dashed line). Expression levels across six life cycle stages, including eggs, first/second-stage larvae (L1/2), infective third-stage larvae (iL3), infective larvae isolated from the host lung (lL3), young PF (yPF) i.e. without eggs, and gravid PF (gPF) i.e. carrying eggs are shown in the chart. Note, PF and gPF samples are both prepared from gravid parasitic females at similar dpi but the library construction methods (amplified and non-amplified) and sequencing run batches and machines (MiSeq and HiSeq) differ, respectively. FPKM = Fragments Per Kilobase per Million mapped reads.