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. 2018 Mar 26;8:5192. doi: 10.1038/s41598-018-23514-z

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Phylogeny of speckle-type POZ-like (SPOP-like) genes. Amino acid sequences for predicted SPOP-like genes from 14 nematodes species, spanning five evolutionary clades (as defined by Blaxter et al.³²), were used to construct a phylogeny. SPOP-like genes are highlighted for S. venezuelensis (red) and S. papillosus (blue); genes for all other species are shown in black. A gene cluster present in all 14 species is shown with a green background highlight (A). Genes upregulated in the transcriptome of the parasitic adult female, compared with the free-living adult female, are shown with background highlighting for S. venezuelensis and S. papillosus (pink background (B), excluding genes shown with a white spot). Scale bar represents average amino acid substitutions per site. *Represents branch support values greater than 70, for 100 bootstraps. Gene names for all genes used to build this phylogenetic tree are shown in Supplementary Fig. S6.