Table 4.

List of HRM primers designed for genotyping polymorphic genic SNPs from each chromosome.

Primer no.	Locus name	Gene name	5′-3′primer sequence	3′-5′primer sequence	Result of HRM analysis validated in F 4 population
1	CA01g00370	Serine/threonine protein kinase%2C putative	CGGCCAATGTATCAAGACTCG	AACGAATTCAACAACCGCGT	Positive
2	CA01g02310	Xpa-binding protein%2C putative	TCCCTTCTGCGGTTTTCCTC	TGTTGCAAACTTCTCCTTGTAGG	Positive
3	CA01g04020	Kinesin heavy chain%2C putative	CCCACTGGTGAAAGCAGTGT	TGGAGAGAAGGCCTCAATGG	Positive
4	CA02g00020	DNA-repair protein UVH3%2C putative	TGGTCAGGTAATGGTGGTTCT	CTCTCCCTCATCTGGCAAACA	Negative
5	CA02g00720	Pentatricopeptide repeat-containing protein%2C putative	AGAGCACTAACCTCTTTAGCA	GACTGCAAAGACCCCACAGA	Positive
6	CA02g02750	MYBR domain class transcription factor	ACAGTCATACTAGATGAAGGCGG	TGATGCAATGTGGTCAGATGA	Negative
7	CA03g00110	Beta-galactosidase	AGTAACTGATGGAATTTCGGAA	TGGATGCGTTTTAGCCTGACT	Positive
8	CA03g00740	Small subunit processome component-like protein	TCCCAGCATACTCGTCCAAC	CCTCAACCTAGGCATGCCAA	Negative
9	CA03g15330	PREDICTED: Golgi to ER traffic protein 4 homolog	TGGTTAGTCTTTCCTAATCCGGT	CTATTTCTTTTTCCATTCCATTGC	Positive
10	CA04g00830	Phosphatidylinositol 4-kinase%2C putative	GGGGGCTAGTCTTCTCTTCT	GGCAACAAGGTGGAAAGACG	Negative
11	CA04g00250	PREDICTED: transmembrane emp24 domain-containing protein p24beta3-like	CGGATCATCCCGGCATTGAT	TCACCTCCGATTCACAACTCA	Negative
12	CA04g00360	Protein transport protein sec. 23%2C putative	GCACGCCCATACCTTGTCAA	ATCAATGCCAAGCCCATCCA	Positive
13	CA05g00010	RNA polymerase II transcription mediators isoform 1	CAACGAGGCTGACCGAAAGA	CTCCACTCGCCCATCTTCTC	Positive
14	CA05g00320	Folylpolyglutamate synthase	GGTGGGGGCTTTTGTCTTCT	ACTACATCTTCTGAGGTAACACC	Negative
15	CA05g15050	PREDICTED: mediator of RNA polymerase II transcription subunit 33A	CCACCGTTTCAATCCCTTGC	ACGTGTCAGGATTCATAAGCT	Positive
16	CA06g00010	Kinesin heavy chain%2C putative	TGAAGCCGCCTCGAATTTCT	AATGAGACTTCGAGGGGCAC	Negative
17	CA06g01280	Myosin XI%2C putative	ATAGACCCCGGCTCAGGAAT	GCAAAGGTAGCTCCACCACT	Positive
18	CA06g01570	PREDICTED: TBC1 domain family member	GGCAGGAAGATACAATAAATGTAC	AGCAGTATCGTGATTTCATTTGGT	Negative
19	CA07g03700	PREDICTED: synaptotagmin-5-like	AGTAAGGTCAAATGTGGAGCCA	AGAACGTTAATACTGGCCATCG	Negative
20	CA07g04200	Transducin family protein	TGCGAACTTAAGGAAAAAGAAGCA	GTAATGCTTGTCGGGAGCCT	Positive
21	CA07g12460	Formin	GGGATAACGCTCTTCCATATGGA	CATGTCTGACAGAGGGTGCA	Negative
22	CA08g00950	Transcription cofactor%2C putative	ACACTGAGATGCATGCACCA	TACCTGGTTTTGGCTGTGTT	Positive
23	CA08g08740	DNA-directed RNA polymerase	ACAACAGGGACATGATTTCATCA	ACACTAAACCCTTCTGTGCACA	Positive
24	CA08g09730	PREDICTED: protein ZINC INDUCED FACILITATOR-LIKE 1-like isoform X3	TGTGTGTCGAAGCAATTGAT	CTGTTGGAAGATTTGTCAATATCA	Positive
25	CA09g00140	O-linked n-acetylglucosamine transferase	CTGCACATAGAATTCTTGCCCA	TGGGATTGTTTCGTGCTTTT	Negative
26	CA09g01180	Vacuolar protein sorting-associated protein	TTGTCCTCCTCCTCAGATGA	ACCACCAGCAAGAACGTCAA	Negative
27	CA09g14940	Beta-amyrin synthase	TGGCACCATTTTTAAACAACA	ACAGTCAGAAGCACACTGTGA	Positive
28	CA10g01250	PREDICTED: heterogeneous nuclear ribonucleoprotein	TGATGAGCTCGGAGGAGTCA	AAGTGGCTGGGATTCAAGGG	Negative
29	CA10g01280	Protein binding protein%2C putative	GGGTGAGTTTCCTAAGAGGTCC	CAAATCACATGGCCAAACGC	Positive
30	CA10g07870	Amidase%2C putative	GCTGCAGCAATGTAATTGGA	CCTCTGACCATCATCGCTGA	Negative
31	CA11g11870	Xanthine dehydrogenase	ACCTTGACTGGTACACTTTTTCA	AGTGATGACGGACAATTGTGT	Positive
32	CA11g15420	Tubulin family protein	GGCCTCATAACACCGTGGAA	TTACCAGCAGCATTGATCGA	Negative
33	CA11g15430	ABA aldehyde oxidase	TTAATGGAGGCTTCAGAGAGA	GCTTGGGACTCTTGAAAGAAGC	Positive
34	CA12g01070	IsoleucyltRNA synthetase%2C putative	ACAACACCCATCGACTTCCC	TGCAGAGCCAGATTTCAGGT	Positive
35	CA12g02370	N-like protein	TGGTGTTTTTCCATTTGCCT	TCTCTAGAACGTAAGGGTATTCA	Negative
36	CA12g22510	PREDICTED: pleiotropic drug resistance protein	ACCGAGTCGAAAGAGGAAGC	AAGGGCAGAGTCGAGCTTTC	Negative