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. 2018 Mar 26;8:5215. doi: 10.1038/s41598-018-23098-8

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Bone remodelling changes after infusion of Sham and osteoporotic donor-derived MSCs. (A–C) Representative images of calcein labelling (A) and parameters of bone formation rates (B,C) in distal femora harvested at D28 post operation. Bars: 50 μm. n = 3 per group. (D,E) ELISA analysis of serum levels of bone formation markers P1NP (D) and OCN (E). n = 6 per group. (F–H) Representative images of TRAP staining (F) and parameters of bone resorption rates (G,H) in distal femora harvested at D28 post operation. Bars: 25 μm. n = 3 per group. (I,J) ELISA analysis of serum levels of bone resorption markers TRAP-5b (I) and CTX-1 (J). n = 6 per group. Data are shown as mean ± SD. *P < 0.05, **P < 0.01 and ***P < 0.001. NS, not significant (P > 0.05). Data were analysed using ANOVA followed by Newman-Keuls post-hoc tests.