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. 2018 Mar 26;8:5216. doi: 10.1038/s41598-018-23577-y

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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FT-IR analysis. The IR spectra of V125 and V125-E8 showed peaks characteristic of polypeptides, including amide I (1656 cm⁻¹) and amide II (1525 cm⁻¹), asymmetric sp3 CH2 (2965 cm⁻¹), symmetric sp3 CH2 (2875 cm⁻¹), and OH (3307 cm⁻¹). The peaks around 2300 cm⁻¹ in CPC with V125 indicates CO₂ or H₂O in the air, which was not removed by background compensation process.