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. 2018 Mar 26;8:5197. doi: 10.1038/s41598-018-23558-1

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Alignment of Group IId and Group IIe WRKYs in T. chinensis and A. thaliana. Full-length WRKYs of Group IId and Group IIe were aligned together since they were considered as IId + e subgroup. WRKY domain was labeled by the solid line. CaMBD (Ca^2 +-dependent CaM-binding domain) and Plant_zf_clust (plant zinc finger cluster, accession number in pfam was PF10533) domain were framed by solid box and dotted box respectively. HSF_DNA-bind (HSF-type DNA-binding, pfam00447) domain of TcWRKY57 was framed by grey box. LxxLL-motif was framed by red box. Sequence alignment was conducted by ClustalW, the figure was generated by DNAMAN 6.0. Different colors indicate sequence similarities: black indicated 100%, purple is 75%, green is 50%, yellow is 33%.