Figure 5.

Effects of ROS on MICAL1‐modulated Akt activity and ERK/cyclin D signalling. (A) Effects of MICAL1 on ROS generation in MCF‐7 and T47D cells. *P < .05 the cells transfected with siMICAL1 relative to the cells with control siRNA. (B) Effects of NAC on ROS generation in MCF‐7 and T47D cells. *P < .05, **P < .01 the NAC‐treated group with relative to the control group. (C) T47D cells transfected with MICAL1 and treated with 2 mmol/L NAC for 1 h, protein levels of p‐Akt, cyclin D were examined. (D) T47D cells transfected with MICAL1 were treated with 1 μmol/L LY294002 for 30 min, and protein levels of p‐ERK, c‐myc, CDK4, cyclin D were examined. (E&F) Edu staining shows the effects of U0126 and LY294002 on proliferation in MICAL1‐transfected MCF‐7 (E) and T47D (F) cells. *P < .05. **P < .01