Figure 6.

CXCR7 participates in CXCL12‐mediated signalling in lung epithelial cells. (a) Protein extract was isolated from MLE‐12 cells, their derived control (bearing control vector, control), and CXCR7 knockdown cell line (bearing CXCR7 shRNA expression construct, CXCR7 short hairpin RNA) following stimulation with 100 ng/ml CXCL12 for the indicated time. Western blotting was performed using the indicated antibodies. Quantitative data are shown in the Supplementary material (Fig. S2). (b, c) Indicated cell lines were loaded with 10 nm/ml Fluo3 and stimulated with 250 ng/ml CXCL12. The changes in relative fluorescence intensity due to calcium influx were assessed. Data are expressed as the mean ± SEM of fluorescence change. Statistical significance was determined by one‐way anova. [Colour figure can be viewed at http://wileyonlinelibrary.com]