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. 2018 Apr 14;7(6):487–494. doi: 10.1002/sctm.17-0258

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© 2018 The Authors stemcellstranslationalmedicine published by Wiley Periodicals, Inc. on behalf of AlphaMed Press

This is an open access article under the terms of the http://creativecommons.org/licenses/by-nc-nd/4.0/ License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non‐commercial and no modifications or adaptations are made.

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Cryopreservation of CSSC in CCG. (A): CSSC embedded in CCG were cryopreserved as described in “Materials and Methods” section. After 2 weeks the thawed gels were stained with Calcein AM vital dye and imaged using confocal microscopy. Projections of a three‐dimensional image stack at 45° (top), 90° (middle), and 180° (bottom) show cells as viable and aggregated into flattened disk‐like spheroids. Scale bar = 200 µm. (B): Thawed cryopreserved CSSC (2500 cells/gel) in CCG were highly effective at suppression of corneal scarring in wound healing experiments similar to that of Figure 6B. Sample n = 6, p < .05. Abbreviations: CCG, compressed collagen gel; CSSC, corneal stromal stem cells.