Figure 6.

Tumor suppressive effect of the miR‐143 on DLD‐1 cell xenografted mice. A, DLD‐1 cells were injected subcutaneously into BALB/c nude mice. When the tumor volume had reached 100 mm³, 8 mice were sorted into each group. Then, control‐miR, #1 or #12 was intravenously injected every 72 h. At the second administration of #12, cetuximab was intraperitoneally injected every 72 h. The tumor volume was calculated as 0.5236 L₁ (L₂)², where L₁ is the long axis and L₂ is the short axis of the tumor. C vs #1; P = .03 (day 16), C vs #12; P = .02 (day 13), C vs #12 + E; P = .05 (day 10), #12 vs #12 + E; P = .04 (day 19). The dose of 750 μg/kg/injection was given intravenously to each treated mouse every 3 d. The protocol is shown in the figure. B, The body weight was measured at every administration time. C, Western blot analysis was performed to determine the levels of K‐Ras, p‐AKT, AKT, p‐ERK1/2, ERK1/2 and ERK5 proteins in the xenografted tumor samples. β‐actin was used as an internal control. *P < .05 vs control (Student's t test)