Figure 2.

S1PR3 is essential for the anti‐inflammatory effects of pFTY720 in astrocytes. Astrocytes were transfected with s1pr3 siRNA (si‐S1PR3) or negative control (NC) for 48 hr and then treated with oxygen‐glucose deprivation (OGD). Expression of S1PR3 (A), cell viability (B), pro‐inflammatory cytokine levels in the cellular supernatant including HMGB1 (C) and TNF‐α (D) was determined. *P < .05, **P < .01 vs Con group; #P < .05 vs OGD group; $P < .05 vs OGD plus pFTY720 pre‐treatment group. Results are shown as mean ± SEM. in every 6 independent experiments. OGD: oxygen‐glucose deprivation; pFTY720: phosphorylated FTY720