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The primers for PCR amplification
| Gene name | Forward (5′ to 3′) | Reverse (5′ to 3′) |
|---|---|---|
| GAPDH | GGAGACAACTGGTCCTCCAGTG | ACCTGCCAAGTATGATGACATCA |
| S1pr1 | TTCAGCCTCCTTGCTATCGC | AGGATGAGGGAGATGACCCAG |
| S1pr2 | GACGCTGGACATGCAGGAG | TACATGGCTGAGTGGAACTT |
| S1pr3 | GCCACCCGCCAGTCTTG | GCCAGCTTCCCCACGTAAT |
| S1pr4 | CGTTTCCAGCATCCGCAG | CCAGTCCCTTCTCACCTCTCCT |
| S1pr5 | CCTATGTGCTCTTCTGCGTGCTG | CGCACCTGACAGTAAATCCTTG |
| S1PR3‐siRNA | CUCCAAAGGUCAAGGAAGATT | UCUUCCUUGACCUUUGGAGTT |
