Figure 2.

ABCC1 was up‐regulated in oxaliplatin‐resistant cells and NR2F1‐AS1 knockdown suppressed the oxaliplatin resistance of hepatocellular carcinoma (HCC) cells. A, The survival percentage of oxaliplatin‐resistant HCC cells (Huh7/OXA, HepG2/OXA) and their parental cells when treated with increasing concentration of oxaliplatin (OXA). B, C, Western blot showed the ABCC1 protein expression in oxaliplatin‐resistant HCC cells (Huh7/OXA, HepG2/OXA) and their parental cells. D, Interfering oligonucleotides were synthesized and transfected into Huh7/OXA and HepG2/OXA cells. E, RT‐PCR showed the mRNA expression levels of drug resistance‐related genes, including MDR1, MRP5, LRP1. F, G, The 50% inhibitory concentration (IC50) value was measure in Huh7/OXA and HepG2/OXA cells. si‐1#, 2#, 3# present “siRNA‐targeting NR2F1‐AS1.” Data were expressed as mean ± SD. *P < .05, **P < .01 represents statistically difference