Figure 4.

The combined action of miR‐140‐5p and DEX dampens neuron apoptosis in HIBD rats. Notes: A, Results of TUNEL staining among groups; B, Histogram of cell apoptosis rate; C, mRNA expressions of apoptosis‐related genes; D, Protein expressions of apoptosis‐related genes; E, Protein bands of apoptosis‐related genes; *, P < .05, compared with the sham group; #, P < .05, compared with the model group; &, P < .05, compared with the DEX group; three surviving rats were randomly selected from the sham and model groups, respectively, and ten surviving rats were selected from other groups, respectively; the experiment was repeated three times; DEX, dexmedetomidine; NC, negative control; miR‐140‐5p, microRNA‐140‐5p; Bcl‐2, B‐cell lymphoma‐2; Bax, Bcl‐2‐associated X protein; TUNEL, terminal deoxynucleotidyl transferase‐mediated dUTP‐biotin nick end labelling