Figure 5.
Wnt1 is verified as one of the target gene of miR‐140‐5p determined by dual‐luciferase reporter gene assay. Notes: A, Sequences in the 3′UTR of Wnt1 mRNA binding with miR‐140‐5p; B, Relative luciferase activity of Wnt l‐3′UTR reporter gene vector; *, P < .05, compared the mutant groups; the experiment was repeated three times; WT, wild type; MT, mutant; miR‐140‐5p, microRNA‐140‐5p