Table 1.

Primer sequences for RT‐qPCR

Target gene	Primer sequence
miR‐140‐5p	Forward: 5′‐CAGTGGTTTTACCCTATGGTAG‐3′
	Reverse: 5′‐ACCATAGGGTAAAACCACTGTT‐3′
U6	Forward: 5′‐GCTTCGGCAGCACATATACTAAAAT‐3′
	Reverse: 5′‐CGCTTCACGAATTTGCGTGTCAT‐3′
Wnt1	Forward: 5′‐GGACTTGCTTCTCTTCTCATAGCC‐3′
	Reverse: 5′‐CCACACAGGCATAGAGTGTCTGC‐3′
β‐catenin	Forward: 5′‐AAAATGGCAGTGCGTTTAG‐3′
	Reverse: 5′‐TTTGAAGGCAGTCTGTCGTA‐3′
E‐cadherin	Forward: 5′‐CGG TGG TCAAAGAGCCC TTAC T‐3′
	Reverse: 5′‐TGAGGG TTGGTGCAACAACG TCGTTA‐3′
TCF‐4	Forward: 5′‐CAGGATCCAAAAGCTGGTG‐3′
	Reverse: 5′‐CCGGATCCTATAAGACGG TGACGAG‐3′
β‐actin	Forward: 5′‐GCACCACACCTTCTACAATGAG‐3′
	Reverse: 5′‐ACAGCCTGGATGGCTACGT‐3′
Bcl‐2	Forward: 5′‐CTGGTGGACAACATCGCTCTG‐3′
	Reverse: 5′‐GGTCTGCTGACCTCACTTGTG‐3′
Bax	Forward: 5′‐CTGAGCTGACCTTGGAGC‐3′
	Reverse: 5′‐GACTCCAGCCACAAAGATG‐3′
Caspase‐3	Forward: 5′‐ATGGACAACAACGAAACCTC‐3′
	Reverse: 5′‐TTAGTGATAAAAGTACAGTTCTT‐3′

miR‐140‐5p, microRNA‐140‐5p; TCF‐4, T cell factor 4; Bcl‐2, B‐cell lymphoma‐2; Bax, Bcl‐2‐associated X protein; RT‐qPCR, reverse‐transcription quantitative polymerase chain reaction.