Official websites use .gov
A
.gov website belongs to an official
government organization in the United States.
Secure .gov websites use HTTPS
A lock (
) or https:// means you've safely
connected to the .gov website. Share sensitive
information only on official, secure websites.
PCR reaction primers in RT‐qPCR
| Genes | Forward primer(5′‐3′) | Reverse primer(5′‐3′) |
|---|---|---|
| TLR4 | GGACTCTGCCCTGCCACCATTTA | CTTGTGCCCTGTGAGGTCGTTGA |
| NF‐κB‐p65 | GTGCAGAAAGAAGACATTGA | AGGCTAGGGTCAGCGTATGG |
| MyD88 | ATAGGCACCAGCATGCAC | TAGGGTCCTTACCAGGTA |
| β‐actin | ACTGGCATTGTGATGGACTC | CAGCACTGTGTTGGCATAGA |
RT‐qPCR, reverse transcription quantitative polymerase chain reaction; TLR4, toll‐like receptor 4; NF‐κB‐p65, nuclear factor‐kappaB‐p65; MyD88, myeloid differentiation factor 88.
