Figure 2.

SNP rs1055259 affects luciferase activities in 786‐O cells. (A) Reporter plasmids construct. The schematic diagrams show the luciferase reporters carrying 2 copies of rs1055259‐A allele or G allele or mutant sequence at the 3′UTR of Luciferase gene. (B) This plot shows the theoretical miRNA‐mRNA duplex pairing between miR‐556 and GSTM3 3′UTR. The A, G alleles and mutant sequence are highlighted with an asterisk (*). (C, D) Relative luciferase activities (vs Renila luciferase) were measured in ccRCC cells expressing either A allele, G allele or mutant construct which were treated with control miR, miR‐556 mimic or antagomir‐miR‐556. (E) After incubated with 50 μmol/L CoCl2 for 24 h, luciferase activities were examined in cells transfected with either A allele, G allele or mutant construct. Six replicates for each group and the experiment were repeated at least 3 times. *P < .05, ns: non‐significant compared between treatment group and control group