Table 2.

Primers for PCR and single‐base extension reaction in MALDI‐TOF MS assay

Gene (GSTM3) Loci	Chr:position	Forward primera	Reverse primera	Extension primer	Amplicon size (bp)
rs1537236	1:109736350	linker‐GCCCATAGGCTGCTCTGC	linker‐ GCCTTAGGAAAAGTTGTAG	CACAAA GGAAAGATTT	121
rs3814309	1:109734781	linker‐GTGGCAAAACATGGCA GTTG	linker‐ CAGGGATACCCAGTGATGTC	TTAGCACCTTCTGTCTG	78
rs1109138	1:109735327	linker‐AGCTTCAAATTCCTGG GCTC	linker‐ ACCCCTGTCTCCCAAAAATT	GGACTATGCATGAGCAA	69
rs1055259	1:109734239	linker‐AGTGACCATCCCAACTTTGG	linker‐ CCATGGGGGTGCTAAAACCAC	TTTAACCCAGCTCATTAT	119
rs5776997	1:109736432	linker‐GGGGTAGGAGAAGTTT CTTT	linker‐ TACTCCACAGGCAGAGCAG	CTTTAAAGACGTAAAAAGAAAAAA	86

^alinker = 10‐mer linker ACGTTGGATG placed at 5′‐end of each primer.