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. 2018 Jan 26;154(2):285–297. doi: 10.1111/imm.12884

Figure 2.

Figure 2

Blockade of CD70 reduces primary expansion of CD8 T cells but not antibody responses after modified vaccinia virus Ankara (MVA) immunization. C57BL/6 mice were immunized intravenously with 5 × 107 TCID 50 recombinant rMVA (rMVA) alone or in combination with 300 μg anti‐CD70 (FR70) intraperitoneally on days 0, 4 and 7. Peripheral blood lymphocytes were gated on CD4− CD8+ CD44+ B820‐27‐dextramer+ T cells and further analysed for CD62L and CD127 expression (a). The frequency of B8‐specific CD8 T cells over time is shown in (b). CD127 expression on B820‐27‐specific CD8 T cells is shown as geometric mean fluorescence intensity (GMFI) (c). The total number (d) and memory phenotype (e) of splenic B820‐27‐specific CD8 T cells was determined on day 84. MVA‐specific IgG titres in the serum on day 21 were determined by ELISA (f). Results are shown as mean ± SEM from four mice per group. Data are representative of at least two independent experiments. **P < 0·005.