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. 2017 Dec 29;175(12):2272–2283. doi: 10.1111/bph.14098

Figure 3.

Figure 3

Effect of GI‐530159 on stably transfected TREK1 channel currents. (A) Whole‐cell current recordings of TREK1 channels stably transfected in HEK293 cells, in the presence (blue) and absence (green) of GI‐530159 (1 μM). (B) Current–voltage relationship for TREK1 currents in the presence (blue) and absence of GI‐530159. The inset shows the current–voltage relationship for GI‐530159‐activated current. (C) Control current, measured at 0 mV (green), was significantly enhanced by 1 μM GI‐530139 (blue, n = 6, *P < 0.05, paired t‐test). Each n value represents a recording from a cell on an independent coverslip on different recording days. (D) Representative single‐channel records of hTREK‐1 in excised inside‐out membrane patches (12 inside‐out patch recordings in total) from HEK293 cells in the presence and absence of GI‐530159 (10 μM). Dotted line indicates the closed channel state, and upward deflections correspond to channel openings. Membrane patches were voltage clamped at +60 mV at room temperature.