Figure 4.

LKB1 promotes Snail degradation through enhancing the binding of Snail and E3 ligase FBXL14. A, Protein expression of Snail in Capan‐2 pBABE transfected with siControl, Capan‐2 wtLKB1 and Capan‐2 wtLKB1, respectively, transfected with siControl and siFBXL14; B,C, Transwell assays were employed to show the migration (B) and invasion (C) of Capan‐2 pBABE,, Capan‐2 wtLKB1 and Capan‐2 pBABE or Capan‐2 wtLKB1 transfected with FBXL14 siRNA for 24 h. Quantitative results are illustrated for panels (right). Data represent the mean ± SD (n = 3) from 3 separate experiments. **P < .01, ***P < .001, Student's t test. Scale bar, 200 μm; D, Immunoprecipitation (IP) analysis to show the interaction between endogenous LKB1 and Snail in PANC‐1; E, Western blot analysis to show the binding between LKB1 and endogenous FBXL14 in PANC‐1; F, Western blot analysis was performed to show the effect of LKB1 on interaction between Snail and endogenous FBXL14