Figure 6.

Levels of total Rb and its phosphorylated form pRb(S780). Western blot analysis demonstrating a time course of 82‐, 91‐ or 95‐dependent inhibition of Rb phosphorylation at S780 in MDA‐MB‐453 breast cancer cell line (A) and MV4‐11 leukaemia cell line (B and C). D. Compound 91‐dependent inhibition of Rb phosphorylation at S780 in T47D and MCF7 breast cancer cell lines. Cells were treated for 4, 12 and/or 24 h with each compound at increasing concentrations, viz. GI₅₀, 5 × GI₅₀ and 10 × GI₅₀ of 82, 91 or 95. The respective GI₅₀ values for each cell line are shown in Tables 1 or 2. β‐actin was used as a loading control. Images representative of two independent experiments are shown. PD: PD0332991.