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. 2018 Apr 27;109(5):1532–1544. doi: 10.1111/cas.13585

Figure 6.

Figure 6

MicroRNA‐19a (miR‐19a) knockdown attenuates the promotion effects of placental growth factor (PLGF) on gallbladder cancer (GBC) cells. A, B, Migration and invasion assays of GBC‐SD cells infected with antagomir‐19a lentivirus or negative control (antagomir‐NC) and treated with or without 100 ng/mL PLGF. A, Representative images. B, Quantitative analysis of the numbers of migrated and invaded cells. C, D, Sphere‐formation assays of GBC‐SD cells infected with antagomir‐19a lentivirus or antagomir‐NC and treated with or without 100 ng/mL PLGF. (C) Representative images. D, Quantitative analysis of the numbers of spheres. *P < .05; **P < .01. E, Immunoblotting analyses of the levels of epithelial–mesenchymal transition (EMT) and cancer stem cell marker proteins in GBC‐SD cells after infection with antagomir‐19a lentivirus or antagomir‐NC and treatment with or without 100 ng/mL PLGF. E‐cad, E‐cadherin; N‐cad, N‐cadherin. F, Working model of the PLGF–c‐Myc–miR‐19a–PTEN regulatory axis in GBC