Table 1.
The 384‐bp synthetic DNA fragments designed based on a sequence encompassing the promoter region of the FAM150A gene
| Sequence | ||
|---|---|---|
| (a) Original sequence of the promoter region of the FAM150A gene | ||
| GGGAGGACCC AGTAGGGTAA CTGCCGCGTC GCCCCGGCGG TTCTCCCTGG GCTCTGTCTC CCGCCGCCTC CACCCCCCGA GCCTCGGGGT CCGTCACGGC TTCCCCTGGC TGGCGGGGTC AGTAGAACCC GCGGCGCCTA GGTCCGGACG GAAAAAAGCA GGGCCGGGGT GCGGCCTGGA TGAGCGGAGA TCTCCGCGCC TTGGGCTCAA AGGTGCGGGG TGCGCTCTGC TGCCGAGCCC CTGCTCGCTC AGGAACACTG GCCACGCCGT CACGCCAGCC GCCCCTGCCC CAGGTCTGGA GGCCCGACCT GCTCTCCTAG GCGCAGCACC GCGTTCTCTT CCGCGTGGGG GAGCGGCGGG CGGAAGAGGT CTGGGGCTGG GCAC | ||
| Synthetic DNA | DNA methylation levels (%) | Sequence |
|---|---|---|
| (b) Sequence of synthetic DNA fragments designed based on the status after bisulfite modification | ||
| Fragment 1 | 0 | GGGAGGATTTAGTAGGGTAATTGT TGTGTTGTTTTGGTGGTTTTTTTTGGGTTTTGTTTTTTGTTGTTTTTATTTTTTGAGTTTTGGGGTTTGTTATGGTTTTT TTTGGTTGGTGGGGTTAGTAGAATTTGTGGTGTTTAGGTTTGGATGGAAAAAAGTAGGGTTGGGGTGTGGTTTGGATGAGTGGAGATTTTTGTGTTTTGGGTTTAAAGGTGTGGGGTGTGTTTTGTTGTTGAGTTTTTGTTTGTTTAGGAATATTGGTTATGTTGTTATGTTAGTTGTTTTTGTTTTAGGTTTGGAG GTTTGATTTGTTTTTTTAGGTGTAGTATTGTGTTTTTTTTTGTGTGGGGGAGTGGTGGGTG GAAGAGGTTTGGGGTTGGGTAT |
| Fragment 2 | 20 | GGGAGGATTTAGTAGGGTAATTGT TGTGTTGTTTCGGTGGTTTTTTTTGGGTTTTGTTTTTTGTTGTTTTTATTTTTTGAGTTTCGGGGTTTGTTATGGTTTTTTTTGGTTGGTGGGGTTAGTAGAATTTG CGGTGTTTAGGTTTGGATGGAAAAAAGTAGGGTTGGGGTGCGGTTTGGATGAGTGGAGATTTTTGTGTTTTGGGTTTA AAGGTGTGGGGTGCGTTTTGTTGTTGAGTTTTTGTTTGTTTAGGAATATTGGTTATGTTGTTACGTTAGTTGTTTTTGTTTTAGGTTTGGAGGTTTGATTT GTTTTTTTAGGTGTAGTATTG CGTTTTTTTTTGTGTGGGGGAGTGGTGGGCG GAAGAGGTTTGGGGTTGGGTAT |
| Fragment 3 | 25 | GGGAGGATTTAGTAGGGTAATTGT TGTGTCGTTTTGGTGGTTTTTTTTGGGTTTTGTTTTTTGTCGTTTTTATTTTTTGAGTTTTGGGGTTTGTTACGGTTTTTT TTGGTTGGTGGGGTTAGTAGAATTTGTGGCGTTTAGGTTTGGATGGAAAAAAGTAGGGTTGGGGTGCGGTTTGGATGAGTGGAGATTTTTGTGTTTTGGGTT TAAAGGTGCGGGGTGTGTTTTGTTGTTGAGTTTTTGTTTGTTTAGGAATATTGGTTACGTTGTTATGTTAGTTGTTTTTGTTTTAGGTTTGGAGGTTCGATTTGT TTTTTTAGGTGTAGTATTGTGTTTTTTTTCG TGTGGGGGAGTGGTGGGCG GAAGAGGTTTGGGGTTGGGTAT |
| Fragment 4 | 30 | GGGAGGATTTAGTAGGGTAATTGT TGTGTCGTTTTGGTGGTTTTTTTTGGGTTTTGTTTTTCGTTGTTTTTATTTTTTGAGTTTCGGGGTTTGTTATGGTTTTTTTTGGTTGGCGGGGTTAGTAGAATTTGTGGCGTTTAGGTTTGGATGGAAAAAAGTAGGGTCGGGGTGTGGTTTGGATGAGTGGAGATTTTCG TGTTTTGGGTTTAAA GGTGTGGGGTGCGTTTTGTTGTTGAGTTTTTGTTTGTTTAGGAATATTGGTTACGTTGTTATGTTAGTCGTTTTTGTTTTAGGTTTGGAGGTTTGATTTGTTT TTTTAGGTGTAGTATCG TGTTTTTTTTTG CGTGGGGGAGTGGTGGGCG GAAGAGGTTTGGGGTTGGGTAT |
| Fragment 5 | 50 | GGGAGGATTTAGTAGGGTAATTGT CG TGTCGTTTTGGCGGTTTTTTTTGGGTTTTGTTTTTTGTCGTTTTTATTTTTTGAGTTTCGGGGTTTGTTACGGTTTTT TTTGGTTGGTGGGGTTAGTAGAATTCG TGGCGTTTAGGTTTGGACGGAAAAAAGTAGGGTTGGGGTGCGGTTTGGATGAGTGGAGATTTTCG TGTTTTG GGTTTAAAGGTGCGGGGTGTGTTTTGTTGTCGAGTTTTTGTTTGTTTAGGAATATTGGTTACGTTGTTACGTTAGTTGTTTTTGTT TTAGGTTTGGAGGTTCGATTTGTTTTTTTAGGTGTAGTATCG TGTTTTTTTTCG TGTGGGGGAGCGGTGGGCG GAAGAGGTTTGGGGTTGGGTAT |
| Fragment 6 | 50 | GGGAGGATTTAGTAGGGTAATTGT CGCGTCGTTTCGGCGGTTTTTTTTGGGTTTTGTTTTTCGTCGTTTTTATTTTTCGAGTTTCGGGGTTCGTTACGGTTTTTTT TGGTTGGCGGGGTTAGTAGAATTCGCGGCGTTTAGGTTCGGACGGAAAAAAGTAGGGTCGGGGTGCGGTTTGGATGAGCGGAGATTTTTGTGTTTTGGGT TTAAAGGTGTGGGGTGTGTTTTGTTGTTGAGTTTTTGTTTGTTTAGGAATATTGGTTATGTTGTTATGTTAGTTGTTTTTGTTTTAGGTTTGGAGGTTTGATTTGT TTTTTTAGGTGTAGTATTGTGTTTTTTTTTGTGTGGGGGAGTGGTGGGTG GAAGAGGTTTGGGGTTGGGTAT |
| Fragment 7 | 50 | GGGAGGATTTAGTAGGGTAATTGT TGTGTTGTTTTGGTGGTTTTTTTTGGGTTTTGTTTTTTGTTGTTTTTATTTTTTGAGTTTTGGGGTTTGTTATGGTTTTTTT TGGTTGGTGGGGTTAGTAGAATTTGTGGTGTTTAGGTTTGGATGGAAAAAAGTAGGGTTGGGGTGTGGTTTGGATGAGCGGAGATTTTCGCG TTTTGGGTTTAAAGGTGCGGGGTGCGTTTTGTTGTCGAGTTTTTGTTCGTTTAGGAATATTGGTTACGTCGTTACGTTAGTCG TTTTTGTTTTAGGTTTGGAGGTTCGATTTGTTTTTTTAGGCGTAGTATCGCGTTTTTTTTCGCGTGGGGGAGCGGCGGGCG GAAGAGGTTTGGGGTTGGGTAT |
| Fragment 8 | 50 | GGGAGGATTTAGTAGGGTAATTGT TGTGTTGTTTTGGTGGTTTTTTTTGGGTTTTGTTTTTTGTTGTTTTTATTTTTTGAGTTTTGGGGTTTGTTACGGTTTTT TTTGGTTGGCGGGGTTAGTAGAATTCGCGGCGTTTAGGTTCGGACGGAAAAAAGTAGGGTCGGGGTGCGGTTTGGATGAGCGGAGATTTTCGCG TTTTGGGTTTAAAGGTGCGGGGTGCGTTTTGTTGTCGAGTTTTTGTTCGTTTAGGAATATTGGTTACGTCGTTACGTTAGTCG TTTTTGTTTTAGGTTTGGAGGTTTGATTTGTTTTTTTAGGTGTAGTATTGTGTTTTTTTTTGTGTGGGGGAGTGGTGGGTG GAAGAGGTTTGGGGTTGGGTAT |
| Fragment 9 | 75 | GGGAGGATTTAGTAGGGTAATTGT CGCGTCGTTTTGGCGGTTTTTTTTGGGTTTTGTTTTTCGTCGTTTTTATTTTTTGAGTTTCGGGGTTCGTTACG GTTTTTTTTGGTTGGTGGGGTTAGTAGAATTCGCGGCGTTTAGGTTTGGACGGAAAAAAGTAGGGTCGGGGTGCGGTTTGGATGAGTGGAGATTTTCGCG TTTTGGGTTTAAAGGTGCGGGGTGTGTTTTGTTGTCGAGTTTTTGTTCGTTTAGGAATATTGGTTACGTTGTTACGTTAGTCGTTTTTGTTTTAGGTTTGGAGGTT CGATTTGTTTTTTTAGGTGTAGTATCGCGTTTTTTTTCG TGTGGGGGAGCGGCGGGCG GAAGAGGTTTGGGGTTGGGTAT |
| Fragment 10 | 100 | GGGAGGATTTAGTAGGGTAATTGT CGCGTCGTTTCGGCGGTTTTTTTTGGGTTTTGTTTTTCGTCGTTTTTATTTTTCGAGTTTCGGGGTTCGTTACGGTTTT TTTTGGTTGGCGGGGTTAGTAGAATTCGCGGCGTTTAGGTTCGGACGGAAAAAAGTAGGGTCGGGGTGCGGTTTGGATGAGCGGAGATTTTCGCG TTTTGGGTTTAAAGGTGCGGGGTGCGTTTTGTTGTCGAGTTTTTGTTCGTTTAGGAATATTGGTTACGTCGTTACG TTAGTCGTTTTTGTTTTAGGTTTGGAGGTTCGATTTGTTTTTTTAGGCGTAGTATCGCGTTTTTTTTCGCGTGGGGGAGCGGCGGGCG GAAGAGGTTTGGGGTTGGGTAT |
Dinucleotides left as CpG even after bisulfite treatment due to methylation are shown in bold and unmethylated and modified dinucleotides (TpG) are underlined. PCR primer positions are double‐underlined.