Skip to main content
. 2018 Feb 15;7(6):e1431086. doi: 10.1080/2162402X.2018.1431086

Figure 4.

Figure 4.

JNK activation is required for spautin-1-induced BAD expression. (A) Western blot analysis of expression of indicated proteins in HCT116 and CT26 cells following treatment with spautin-1 (10 uM) for 24 hours. (B, C) Indicated cells were treated with spautin-1 (10 uM) in the absence or presence of SP600125 (500 nM), CC-401 (100 nM), SB203580 (1 uM), SB239063 (100 nM), SCH772984 (100 nM), and LY3214996 (50 nM) for 24 hours. Bad mRNA and cell viability were assayed (n = 3, *p < 0.05, unpaired t-test). (D) Western blot analysis of expression of indicated proteins in HCT116 and CT26 cells following treatment with spautin-1 (10 uM) in the absence or presence of SP600125 (500 nM) or CC-401 (100 nM) for 24 hours. (E) Q-PCR analysis of Jun gene expression in indicated Jun knockdown cancer cells (n = 3, *p < 0.05 versus control shRNA group, unpaired t-test). (F-I) Knockdown of Jun inhibited spautin-1-(10µM) induced Bad mRNA expression (F), cell death (G), Bad promoter activity (H), and CASP3 activity (I) in indicated cells (n = 3, *p < 0.05 versus control shRNA group, unpaired t-test).