Figure 1.

CD11a and EPCR inclusion enriches for HSCs within KLS population. (A): Representative sorting scheme of CD11a– EPCR+ KLS (green) and CD11a+ KLS (orange) populations from Kit‐enriched CFP+ BM. Each sorted population (1,500 CD11a– EPCR+ cells or 10,000 CD11a+ cells per recipient) was transplanted into lethally irradiated B6 recipients along with 500,000 Tomato+ WBM helper cells. (B, C): Time‐course analysis of donor chimerism in blood. Total (B) and granulocyte (C) blood chimerism from CD11a– EPCR+ KLS (“CD11a‐EPCR+”) and CD11a+ KLS (“CD11a+”) sources in primary recipients at weeks (W) 4, 8, and 12 post‐transplant. Total blood was defined as CD45+ and granulocytes as CD45+ Gr1+ Mac‐1+. (D): Donor chimerism of HSPCs in the BM of primary recipients 13 weeks post‐transplant. HSPCs are defined as Ter119– CD27+ Sca‐1+ Kit+. (E): Blood granulocyte chimerism in secondary recipients 6 weeks post‐secondary transplant. Secondary transplants were done using 1 × 10⁶ WBM harvested from primary recipients that received “CD11a‐EPCR+” or “CD11a+” donor cells. *, p ≤ .05; **, p ≤ .01; ***, p ≤ .001 (Student's unpaired t test). Abbreviations: BM, bone marrow, EPCR, endothelial protein C receptor; HSPC, hematopoietic stem and progenitor cells.