Figure 4.

Anticancer effect of hispidulin against colorectal cancer (CRC). A, HT‐29 and SW480 cells were treated with hispidulin (10 or 20 μmol/L) for 24 and 48 hours, the antigrowth effect of hispidulin on CRC cells was measured by CCK‐8 assay. B, Cell apoptosis was quantified by flow cytometry after CRC cells were treated with different concentrations of hispidulin (10 or 20 μmol/L) for 24 hours. C, Effect of hispidulin on activation of caspase‐3 and poly(ADP‐ribose) polymerase (PARP). D, After cells were incubated with hispidulin for 24 hours, the effect of hispidulin (10 or 20 μmol/L) on cell invasion ability was analyzed using Transwell invasion assay. E, HT‐29 and SW480 cells were treated with hispidulin (10 or 20 μmol/L) for 24 h. Western blot analysis was used to detect the expression of MMP‐2 and MMP‐9 using indicated the antibodies. **P < .01