Table 2.
Groups of cases that displayed common CDR3 amino acid sequences. Three distinct clonotypes (identical TRBV-TRBJ genes and CDR3 amino acid sequence) were shared between cases #6 and #16, #12 and #22 and #7 and #16. Case #1 showed the same CDR3 but different TRBV gene than cases #7 and #16. A remarkable HLA restriction was observed between cases harboring identical CDR3; similarities are highlighted in bold. #Purity of CD4+ cells: 69%.
| T cell | TRBV-D-J gene rearrangement |
Shared CDR3 | HLA haplotype |
|||||||
|---|---|---|---|---|---|---|---|---|---|---|
| Case ID | Diagnosis | fraction | TRBV | TRBD | TRBJ | amino acid sequence | HLA-A | HLA-B | HLA-C | HLA-DRB1 |
| #6 | MBL | CD8+ | TRBV6-5 | TRBD1 | TRBJ1-5 | ASSHGGSNQPQH | 02, 11 | 18, 44 | 12, 16 | 11:01, 11:04 |
| #16 | MBL | CD8+ | 02, 30 | 07, 18 | 05, 07 | 03:01, 07:01 | ||||
| #12 | MBL | CD4+ | TRBV6-5 | TRBD1 | TRBJ2-2 | ASSYSGTGNTGELF | 03, 11 | 35, 44 | 12, 16 | 04:08, 07:01 |
| #22 | CLL | CD8+ | 24, 33 | 14, 44 | 08, 08 | 01:02, 07:01 | ||||
| #1 | MBL | CD4+ | TRBV27 | TRBD2 | TRBJ1-5 | ASSLEGDQPQH | 02, 30 | 18, 49 | 05, 06 | 03:01, 11:04 |
| #7# | MBL | CD4+ | TRBV5-1 | TRBD2 | 24, 25 | 07, 07 | 07, 07 | 01:01, 01:01 | ||
| #16 | MBL | CD8+ | TRBV5-1 | TRBD1 | 02, 30 | 07, 18 | 05, 07 | 03:01, 07:01 | ||