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ELISA analysis of YKL-39 secretion in long-term macrophage cultures. Human CD14+ monocytes, non-stimulated (NS) or stimulated with IL4 or IL4+TGF-beta were cultured for 6 and 12days. Highest levels of secreted YKL-39 were detected in the conditioned medium of IL4+TGF-beta stimulated macrophages on day 12. For the statistical analysis Student's paired t-test was used (* p < 0.05). Error bars represent SE.
