Figure 5.

THP‐1‐derived macrophages were recruited by periostin (POSTN) by A2780 cell conditioned medium (CM). A, Representative images of THP‐1‐derived macrophages (Mφ) migrating to A2780 cell‐derived CM. OVCAR‐3 cells were used as a negative control for ovarian cancer‐derived POSTN. B, CM of A2780 cells transfected by control or POSTN siRNA were used for Transwell assay. C, Representative images of THP‐1‐derived macrophages migrating to A2780 cell‐derived CM pretreated with or without POSTN neutralizing antibody (NAb). D, Malignant ascites (n = 3) were pretreated with 2.5 μg/mL POSTN NAb or isotype (iso) Ab. Ascites were applied to the Transwell assay and the number of THP‐1‐derived macrophages recruited by ascetic fluids was counted