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. 2017 Jul 30;175(12):2261–2271. doi: 10.1111/bph.13935

Figure 6.

Figure 6

The effect of carbamazepine is not mediated via a use‐dependent inhibition of Nav1.7‐I234T mutant channels. The use‐dependent block, defined as the ratio of the peak from the 30th pulse normalized to the peak of the first pulse, at a frequency of 20 Hz recording, is determined for each cell. (A, B) Representative first and last (30th) current traces recorded from HEK293 cells expressing Nav1.7‐WT channels with either (A) DMSO or (B) carbamazepine (CBZ) pre‐incubation. (C, D) Representative first and last (30th) current traces recorded from HEK293 cells expressing Nav1.7‐I234T mutant channels with either DMSO (C) or carbamazepine (D) pre‐incubation. (E, F) Use‐dependent block curves of Nav1.7‐WT and Nav1.7‐I234T mutant channels at a frequency of 20 Hz recording were plotted. (E) Use‐dependent fall‐off of peak Nav1.7‐WT current with the pre‐incubation of DMSO or 30 μM carbamazepine.*P <0.05, significantly different from DMSO; n=9. (F) Use‐dependent fall‐off of peak Nav1.7‐I234T current with the pre‐incubation of DMSO or 30 μM carbamazepine.