Figure 2.

Pertussis toxin attenuates ADP‐evoked intracellular Ca²⁺ responses in THP‐1 cells. (A) Averaged (n = 5) intracellular Ca²⁺ response evoked by ADP (3 μM) in the absence (closed circles) and presence (open circles) of 5 nM pertussis toxin (3 h pre‐incubation). (B) ADP concentration–response relationship in absence and presence of 5 nM pertussis toxin (n = 5). (C) Positive control showing abolition of control CCL2‐evoked response by 5 nM pertussis toxin (n = 5). Responses evoked by 50 ng·mL⁻¹ CCL2. For all experiments, F ratio is the ratiometric measurement of intracellular Ca²⁺ using fura‐2. *P < 0.05.