Figure 5.

Inhibition of adenylate cyclase reverses antagonistic action of ticagrelor on ADP‐evoked Ca²⁺ responses in THP‐1 cells. (A) Averaged (n = 5) 3 μM ADP‐evoked Ca²⁺ in the presence of vehicle (closed circles) or following adenylate cyclase inhibition with 300 μM SQ22536. (B) Averaged (n = 5) 3 μM ADP‐evoked Ca²⁺ in the presence of vehicle, 5 μM ticagrelor (open circles) or 5 μM ticagrelor plus 300 μM SQ22536 (closed squares). (C) ADP concentration–response curve (n = 5) in the presence of vehicle (closed circles), 5 μM ticagrelor (open circles) or 5 μM ticagrelor plus 300 μM SQ22536 (closed squares). For all experiments, F ratio is the ratiometric measurement of intracellular Ca²⁺ using fura‐2. *P < 0.05.