FIG. 10.
Brain uptake index (BUI) correlation between in vivo and in vitro. (a) In vitro model using human brain capillary endothelial cells and (b) Caco-2 cell line (colon-epithelial cell line). Reproduced with permission from Kim et al., Biomicrofluidics 9, 1–15 (2015).Copyright 2015 AIP publishing.123 (c) Vascular permeability measurement after mannitol disruption and (d) average normalized variation in fluorescence intensities (ΔF/F0) of X-Rhod-1 that measures calcium concentrations and consequently reduced glutamate transport across the endothelial barrier. Reproduced with permission from Adriani et al., Lab Chip 17, 448–459 (2017). Copyright 2016 Royal Society of Chemistry.86