Figure 1.

(A) Absorbance at 450 nm was tested every minute for 15 minutes to determine the activity of lysozyme. The highest activity was in the folded lysozyme sample followed by typcial FPOP control, quench condition, over‐oxidized control, typical FPOP sample, over‐oxidized sample, and unfolded sample, respectively. (B) For each condition, the activity was normallized using their protein concentration and compared to the folded lysozyme, which was set to 100% activity. (C) Extent of FPOP modifications for lysozyme on the residue‐level for the typical FPOP and over‐oxidization condition. Red boxes highlight the residues involved with catalytic activity and purple boxes highlight the buried residues.