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. 2017 Jul 21;175(12):2284–2295. doi: 10.1111/bph.13910

Figure 4.

Figure 4

MONIRO‐1 block of human Cav2.2 channels. (A) (i) Fraction blocked and (ii) recovery from block by 30 μM MONIRO‐1, with a depolarizing pulse duration of 45 ms or 135 ms, from a holding potential of −80 mV to a test potential that elicits maximal inward current, repeated every 10 s. The number of cells is indicated in parentheses. (B) Fraction of current inhibited by 10 μM MONIRO‐1 at three different frequencies (0.03, 0.1 and 0.2 Hz; holding potential −80 mV). The number of cells is indicated in parentheses. (C) Effect of holding potential (−100, −80 and −60 mV) on the fraction of current blocked by 30 μM MONIRO‐1 (pulsed at 0.1 Hz). The number of cells is indicated in parentheses. (D) Activation and SSI curves in the absence (control) and presence of 30 μM MONIRO‐1. Activation curves were generated through indicated depolarizing test pulses (135 ms) from a holding potential of −80 mV, repeated every 10 s. For SSI curves, selected pre‐pulse potentials (−120 to 0 mV in 5 mV increments for 10 s) were applied prior to a test pulse (135 ms) determined from the peak current, repeated every 15 s from a holding potential of −80 mV. (E) Recovery from inactivation in the absence (control) and presence of 30 μM MONIRO‐1. A two‐pulse protocol (Inset) was repeated every 15 s, where the time was varied between the first fully inactivating pulse and the test pulse (0–10 s). *P < 0.05, significantly different as indicated; n.s. = not significant; Student's t‐test.