Table 1.

Assay Details Help Explain Differences in ΔG_u Results for Wild‐Type Gβ1

Study	Reference	ΔG u (kcal/mol)a	Techniqueb	T (°C)c	pH
57	Choi and Mayo32	5.9d	Thermal denaturation, circular dichroism	25	5.5
61	Gronenborn et al.29	5.6	GdmCl denaturation, fluorescence	25	5.4
72	Frank et al.30	4.1	Urea denaturation, fluorescence	25	2.0
74	Kuszewski et al.31	4.8	GdmCl denaturation, fluorescence	5	4.0
171	Davey et al.33	4.1	GdmCl denaturation, fluorescence	25	–e

^aΔG _u, Gibbs free energy of unfolding.

^bGdmCl, guanidinium chloride.

^cT, temperature.

^dValue was −5.9 for ΔG of folding; ΔG _u is therefore opposite in sign (5.9).

^epH not reported.