Figure 1.

Structures of ARBs and AT₁ receptors. (A) The chemical structures of losartan, valsartan, candesartan, irbesartan, telmisartan and eprosartan. Four of the six ARBs exhibit a common structure, namely, a biphenyl‐tetrazole moiety, while telmisartan and eprosartan are non‐biphenyl‐tetrazole ARBs. (B) Secondary structure model of rat AT₁ receptors, revised on the basis of the crystal structure of human AT₁ receptors. Residues that are numbered and highlighted in yellow indicate residues mutated in this study. The epitope tag attached to the C‐terminus in underlined and allowed for detection by the ID4 monoclonal antibody.