Fig. 8.
Robo LOF increases BrdU incorporation in trunk NCC.
Chicken neural tubes (HH14–15) were electroporated with control-GFP or RoboD2-GFP (RD2) plasmids, isolated and cultured for 24 h. A–C shows GFP (green), HNK1 (fuchsia) and BrdU (red nuclei) staining of cells under control conditions (A) or RD2 (B–C). Panels A′, B′, C′ shows the BrdU channel only.