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. Author manuscript; available in PMC: 2019 Jun 1.
Published in final edited form as: Cell Microbiol. 2018 Mar 23;20(6):e12830. doi: 10.1111/cmi.12830

Figure 4.

Figure 4

Figure 4

Figure 4

Figure 4

Figure 4

Effects of C. parvum infection on the mRNA and protein levels of occludin, claudin 2, claudin 4 and E-cadherin in mouse ileum and jejunum. (A) Relative mRNA abundance of occludin, claudin 2, claudin 4 and E-cadherin in total RNA samples extracted from scrapped mucosa of jejunum and ileum of control or 24 and 48 hr C. parvum infected mice was determined by real-time RT-PCR using gene-specific primers. GAPDH was used as an internal control. Values are mean ± SE. (N = 8; *P<0.05 vs. control, **P<0.001 vs. control). (B) Upper panels: Lysates prepared from scrapped mucosa of ileum of control or 24 hr C. parvum infected mice were subjected to SDS-PAGE and probed with occludin and claudin 4 antibodies. Lower panels: Densitometric analysis of relative band intensities with GAPDH as internal control. (C) Immunofluorescence staining of mucosal sections of ileum of control and 24 hr C. parvum infected mice for occludin, claudin 4 and E-cadherin (red), villin (green) and DAPI (blue). Representative images of 4–5 independent experiments are shown.