Phase contrast images of DPSCs at Days 1, 3, and 5 cultured on 3 kPa hydrogel (a–c respectively), 10 kPa hydrogel (d–f respectively), 45 kPa hydrogel (g–i respectively), 75 kPa hydrogel (j–l respectively) and glass in MSCGM (m–o respectively). This figure shows that the cells did not undergo a morphology change (i.e. maintained typical triangular morphology common among stromal cells) over Days 1–5 on the 10 kPa, 45 kPa, 75 kPa or glass substrates, but did undergo elongation on the relatively softer 3 kPa substrate. (All scale bars are 100 μm.)