Figure 2.

Confocal images of DPSCs on glass (a–c), 75 kPa hydrogel (d–f), 45 kPa hydrogel (g–i), 10 kPa hydrogel (j–l), and 3 kPa hydrogel (m–o) for each media condition at Day 7. The green color indicates actin filaments, while the purple color indicates osteopontin (OPN) within the cells. OPN was evident in all cultures by Day 7, as noted by the purple coloring seen in all images, typically in and around the center of the cell. (All scale bars are 50 μm.)