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. Author manuscript; available in PMC: 2019 Jul 1.
Published in final edited form as: J Biomed Mater Res A. 2018 Mar 6;106(7):1789–1797. doi: 10.1002/jbm.a.36382

Figure 5.

Figure 5

Alizarin red staining of cells on the 3 kPa, 10 kPa and glass substrates. Red staining in the images indicates Ca2+ (mineralization), while green staining indicates collagen. The areas where cells tended to become confluent quickly, such as in gel defects or along the edges of the gel, were the only areas with significant mineralization.