Skip to main content
. Author manuscript; available in PMC: 2019 Jun 1.
Published in final edited form as: Cell Microbiol. 2018 Feb 12;20(6):e12829. doi: 10.1111/cmi.12829

Figure 2. F. alocis survival inside human neutrophils.

Figure 2

Neutrophils were challenged with opsonized F. alocis (MOI 10, for 0.5-2-4 h). Viable (green) and non-viable (red/orange) F. alocis was distinguished using BacLight DNA dyes SYTO9 and propidium iodide (PI). A. Representative confocal image where white dashed arrows indicate viable F. alocis (green) and white arrows indicate non-viable (red/orange). N: neutrophil nucleus. B. The percent of viable internalized (attached to and internalized by neutrophils) F. alocis was quantified from 100 neutrophils, respectively. Data are expressed as the mean ± SEM of the percent of viable F. alocis attached to and internalized; from 3 independent experiments. C. TEM images of neutrophils challenged with opsonized F. alocis for 2-4-6-20 h. White dashed arrows indicate phagosomes containing electron dense F. alocis and white arrows indicate phagosomes containing electron-lucent bacteria. Magnification shown is 9800 x. ns= non-significant