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. Author manuscript; available in PMC: 2019 Jun 1.
Published in final edited form as: Mol Microbiol. 2018 Apr 6;108(5):495–504. doi: 10.1111/mmi.13953

Figure 1. Western blots and western dot blots of Superdex-200 column fractions.

Figure 1

(Sections from separate blots were approximated to display all the required fractions. NusG/NusG F165A and ribosome/mutant ribosome preparations were used as controls on all western blots even if not displayed)
  1. 70S ribosome elution profile. Fractions 11–23.
  2. Wild type NusG elution profile. Fractions 11–23 and 31–41.
  3. NusG and 70S (5:1) complex. Fractions 15–23 probed for ribosomes and NusG.
  4. NusG F165A and 70S (5:1) combination. Fractions 15–23 and 31–36 probed for NusG.
  5. NusG and mutant 70S (5:1) combination. Fractions 15–23 and 31–36 probed for NusG.
  6. NusG F165A and mutant 70S (5:1) combination. Fractions 15–23 probed for ribosomes and NusG, fractions 31–36 probed for NusG.
  7. NusG F165A and mutant 70S (10:1) combination. Fractions 15–23 and 31–36 probed for NusG.