Colony-forming ability was assayed in the presence of 3 µg
ml−1 chloramphenicol (Cm) targeting translation elongation and 200
µg ml−1 kasugamycin (Kas) targeting translation initiation. (A)
The effect of nusE M88/D97A and other S10 mutations on strain growth in
the presence of antibiotics. In every strain the nusE gene is linked to
tetracycline-resistant Tn10. The strain order from top to bottom: 10401,
KM821, 11601, KM820, 10400, KM812. (B) The effect of nusG F165A mutation
on strain growth in the presence of antibiotics targeting translation. In all strains the
nusG gene is linked to a kanamycin resistance cassette. The order of
strains from top to bottom: KM848, KM852, KM850, KM854.