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. Author manuscript; available in PMC: 2019 Jun 1.
Published in final edited form as: Mol Microbiol. 2018 Apr 6;108(5):495–504. doi: 10.1111/mmi.13953

Figure 4. Mutations in the NusG:ribosome interface affect transcription-translation coupling in vivo.

Figure 4

Colony-forming ability was assayed in the presence of 3 µg ml−1 chloramphenicol (Cm) targeting translation elongation and 200 µg ml−1 kasugamycin (Kas) targeting translation initiation. (A) The effect of nusE M88/D97A and other S10 mutations on strain growth in the presence of antibiotics. In every strain the nusE gene is linked to tetracycline-resistant Tn10. The strain order from top to bottom: 10401, KM821, 11601, KM820, 10400, KM812. (B) The effect of nusG F165A mutation on strain growth in the presence of antibiotics targeting translation. In all strains the nusG gene is linked to a kanamycin resistance cassette. The order of strains from top to bottom: KM848, KM852, KM850, KM854.